Biological Activity

Resveratrol is a phenolic phytoalexin found in grape skin and at least 72 other plant species that is involved in a diverse range of biological activities (and targets) with anti-inflammatory, cardiovascular protective, cancer chemopreventive, among other beneficial properties. ^[1,2] It was also found to increase CRISPR-mediated HDR efficiency, but more research is needed to understand its underlying mechanism. ^[3]

Successful Applications in CRISPR

(i) Li and colleagues found that resveratrol increased the expression of several CRISPR/Cas9-mediated HDR associated genes including RAD51 in porcine fetal fibroblasts. Results from an eGFP reporter assay demonstrated that HDR efficiency was increased by approximately 3-fold. Maximal effects were achieved with 50 μM of resveratrol. ^[3] * The authors note that resveratrol demonstrated cytotoxic action in a dosage-dependent manner and that it could arrest cells at the S phase. (ii) The effect of resveratrol on HDR-mediated (large fragment) knock-in was also examined in primary porcine

Reference:

1. Szewczuk, L. M., Forti, L., Stivala, L. A., & Penning, T. M. (2004). Resveratrol is a peroxidase-mediated inactivator of COX-1 but not COX-2: a mechanistic approach to the design of COX-1 selective agents. The Journal of biological chemistry, 279(21), 22727–22737. https://doi.org/10.1074/jbc.M314302200
2. Pirola, L., & Fröjdö, S. (2008). Resveratrol: one molecule, many targets. IUBMB life, 60(5), 323–332. https://doi.org/10.1002/iub.47
3. Li, G., Zhang, X., Zhong, C., Mo, J., Quan, R., Yang, J., Liu, D., Li, Z., Yang, H., & Wu, Z. (2017). Small molecules enhance CRISPR/Cas9-mediated homology-directed genome editing in primary cells. Scientific reports, 7(1), 8943. https://doi.org/10.1038/s41598-017-09306-x
4. Linardic, C. M., Jayadev, S., & Hannun, Y. A. (1996). Activation of the sphingomyelin cycle by brefeldin A: effects of brefeldin A on differentiation and implications for a role for ceramide in regulation of protein trafficking. Cell growth & differentiation: the molecular biology journal of the American Association for Cancer Research, 7(6), 765–774.
5. Jang, M., Cai, L., Udeani, G. O., Slowing, K. V., Thomas, C. F., Beecher, C. W., Fong, H. H., Farnsworth, N. R., Kinghorn, A. D., Mehta, R. G., Moon, R. C., & Pezzuto, J. M. (1997). Cancer chemopreventive activity of resveratrol, a natural product derived from grapes. Science (New York, N.Y.), 275(5297), 218–220. https://doi.org/10.1126/science.275.5297.218
6. Maccarrone, M., Lorenzon, T., Guerrieri, P., Agro, A. F. (1999). Resveratrol prevents apoptosis in K562 cells by inhibiting lipoxygenase and cyclooxygenase activity. European Journal of Biochemisty, 265(1), 27-34. https://doi.org/10.1046/j.1432-1327.1999.00630.x

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392803 Resveratrol, 98% [CAS: 501-36-0]